Ht at 4 and subsequently with horseradish peroxidase (HRP)-conjugated goat anti-mouse
Ht at 4 and subsequently with horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:4,000 dilution; Cappel, West Chester, PA, USA) for two h. Signals have been detected making use of West-Q…