Olog pairs (P450s, GSTs, CCEs). (DOCX) File S2 Multiple alignment of B. oleae P450, GST, CCESupporting InformationFigure SBlast statistics. (A) E-value and (B) percentagesimilarity. (TIF)Figure S2 Phylogenetic evaluation of B. oleae putative ABC transporters (subfamily B). Four B. oleae ABC sequences are clustered in B subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Bmor: Bombyx mori, [42] Dpul: Daphnia pulex [43], Dmel: Drosophila melanogaster, Hsap: Homo sapiens, Tcas: Tribolium castaneum. (TIF)Phylogenetic evaluation of B. oleae putative ABC transporters (subfamily D). A single B. oleae ABC sequence is clustered in D subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Dmel: Drosophila melanogaster, Dpul: Daphnia pulex, Hsap: Homo sapiens. (TIF)Figure S3 Figure S4 Phylogenetic analysis of B. oleae putative ABC transporters (subfamilies E and F). 1 B. oleae ABC sequence is clustered in E and one in F subfamily. Agam: Anopheles gambiae, Amel: Apis mellifera, Bmor: Bombyx mori, Dmel: Drosophila melanogaster, Dpul: Daphnia pulex, Hsap: Homo sapiens, Tcas: Tribolium castaneum. (TIF) Table S1 Top BLAST hits in the NCBI nr database. Only contigs having a blast hit are presented. (XLSX) Table S2 Gene ontology categorization benefits. Onlycontigs which returned a single or extra GO terms are presented. (XLSX)Table S3 Distribution of putative enzymes in KEGGpathways. (XLSX)Table S4 Sequence name, nucleotide and amino acidand ABC transporters (B, D, E and F subfamilies) protein sequences using a representative dataset of their counterparts in other species. Aaeg: Aedes aegypti, Acal: Anisopteromalus calandrae, Aech: Acromyrmex echinatior, Agam: Anopheles gambiae, Agos: Aphis gossypii, Amel: Apis mellifera, Apol: Antheraea polyphemus, Bdor: Bactrocera dorsalis (“JF” sequences obtained from [12]), Bger: Blatella germanica, Bmor: Bombyx mori, Bole: Bactrocera oleae, Bpap: Bactrocera papaya Btab: Bemisia tabaci, Cfum: Choristoneura fumiferana, Cpip: Culex pipiens, Ctar: Culex tarsalis, Ctri: Culex tritaeniorhynchus, Dmel: Drosophila melanogaster, Dpas:Depressaria pastinacella, Dpul: Daphnia pulex, Dpun: Diploptera punctata, Harm: Helicoverpa armigera, Hirr: Haematobia irritans, Hsap: Homo sapiens, Hvir: Heliothis virescens, Hzea: Helicoverpa zea, Lcup: Lucilia cuprina, Ldec: Leptinotarsa decemlineata, Mdom: Musca domestica, Mper: Myzus persicae, Ncin: Nephotettix cincticeps, Nlug: Nilaparvata lugens, Ppol: Papilio polyxenes, Sgra: Schizaphis graminum, Tcas: Tribolium castaneum, Tmol: Tenebrio molitor.3-Bromopyridazine Chemical name (ZIP)Acknowledgments“We thank Aris Ilias (University of Crete) for his assist with the cloning and sequencing of P450s.870196-80-8 structure length and sequence, clade and clade identification system of contigs encoding for putative B.PMID:24220671 oleae P450s. (XLSX) Sequence name, accession quantity, nucleotide and amino acid length and sequence, clade and clade identification method of B. oleae P450s obtained by degenerate PCR. (XLSX)Table SAuthor ContributionsConceived and made the experiments: NP JV. Performed the experiments: NP WD SR AC. Analyzed the data: NP WD SR Television. Contributed reagents/materials/analysis tools: AC SR. Wrote the paper: NP WP JV. Developed the software program used in analysis: SR.
HHS Public AccessAuthor manuscriptNature. Author manuscript; available in PMC 2018 April 25.Published in final edited kind as: Nature. 2017 November 23; 551(7681): 464?71. doi:10.1038/nature24644.Author Manuscript Author Manuscript Author Manuscript Author ManuscriptProgrammable base editing.
