E blot was then incubated with a horseradish peroxidase-conjugated anti-Goat antibody
E blot was then incubated using a horseradish peroxidase-conjugated anti-Goat antibody (GE Healthcare; Waukesha, WI) for 1 hour at room temperature. The Optiblot ECL detection kit (Abcam; Cambridge, MA) was…